â-carbolines and Vitamin E Protect Against Nitric Oxide-induced Lipid Peroxidation in Rat Brain Homogenates
نویسنده
چکیده
Tryptoline and pinoline are two â-carbolines isolated from the nervous system of mammals. We have investigated the level of lipid peroxidation (LPO) in rat brain homogenates in the presence of nitric oxide (NO) which was released by the addition of sodium nitroprusside ( SNP ).We also examined the effect of other known antioxidants (vitamin E) on the NO-induced LPO. The concentration of malonaldehyde (MDA) plus 4-hydroxyalkenals (4HAD) was used as an index of LPO.Incubation of (tryptoline, pinoline or vitamin E) with SNP (5 mM) increased MDA+4 HDA production in brain homogenates which were totally prevented by tryptoline, pinoline and vitamin E in a concentration dependent manner. Under the in vitro conditions of this experiment, vitamin E was more efficient than â-carbolines (pinoline and tryptoline) in limiting NO-induced LPO in rat brain homogenates.
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